Dotplot of Gene Expression by Metadata — dotPlotMet • SCWorkflow

This function uses the Dotplot function from Seurat and plots average gene expression values and percent expressed for a set of genes.

dotPlotMet(
  object,
  metadata,
  cells,
  markers,
  plot.reverse = FALSE,
  cell.reverse.sort = FALSE,
  dot.color = "darkblue"
)

Arguments

object: Seurat Object
metadata: Metadata column in Seurat Object to plot
cells: Vector of metadata category factors to plot and should be found in metadata column. Order of plotting will follow exact order as entered.
markers: Vector of genes to plot. Order of plotting will follow exact order as entered
plot.reverse: If TRUE, set metadata categories to x-axis and genes to y-axis (default is FALSE)
cell.reverse.sort: If TRUE, Reverse plot order of metadata category factors (default is FALSE)
dot.color: Dot color (default is "dark blue")

Dotplot with markers and cell types.

This method provides a dotplot showing the percent frequency of gene-positive cells as size of dot and degree of expression as color of dot.